An injectable, self-healing, 3D printable, double network co-enzymatically crosslinked hydrogel using marine poly- and oligo-saccharides for wound healing application
•A biocompatible hydrogel was designed via Synergy of phenolated polyelectrolyte complex (PHEC) and co-enzyme mediated crosslinking.•Chitooligosacharides (COS) incorporation intensified the electrostatic interaction and enhanced biological activities.•The Gel-COS hydrogels exhibited toughness, self-...
Gespeichert in:
Veröffentlicht in: | Applied materials today 2022-12, Vol.29, p.101581, Article 101581 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | •A biocompatible hydrogel was designed via Synergy of phenolated polyelectrolyte complex (PHEC) and co-enzyme mediated crosslinking.•Chitooligosacharides (COS) incorporation intensified the electrostatic interaction and enhanced biological activities.•The Gel-COS hydrogels exhibited toughness, self-healing, moldability, injectability, and 3D printability.•The Gel-COS hydrogel demonstrated enhanced angiogenesis and accelerated wound healing in a full-thickness skin defect.
In this study, we designed dual network hydrogels with antioxidant and antibacterial activities using marine poly- and oligosaccharides with skin wound healing potential. The synergy between dual enzymatic co-crosslinking based on glucose oxidize (GOx)/horseradish peroxidase (HRP) and electrostatic interaction between positively charged chitooligosaccharides (COS) and phenolated chitosan with negatively charged phenolated alginate formed a hydrogel. The Gel-COS hydrogels exhibited toughness, self-healing, moldability, injectability, and 3D printability. Investigation of the physicochemical properties of the hydrogels exhibited a swelling ratio (< 50%) and in vitro biodegradation after 9 days. Furthermore, the hydrogels exhibited antioxidant properties and antibacterial activity against E. coli and S. aureus. The hydrogels were not cytotoxic and enhanced the migration of 3D cell encapsulated 3T3-L1 fibroblasts, blood vessel formation, as well as in vivo wound healing in a rat model. The Gel-COS hydrogel can be considered a promising skin wound dressing material.
[Display omitted] |
---|---|
ISSN: | 2352-9407 2352-9415 |
DOI: | 10.1016/j.apmt.2022.101581 |