The diagnostic applicability of A-type Lamin in non-muscle invasive bladder cancer

Lamin A is a major component of the nuclear lamina maintaining nuclear integrity, regulation of gene expression, cell proliferation, and apoptosis. Its deregulation in cancer has been recently reported to be associated with its prognosis. However, its clinical significance in non-muscle invasive bladder cancer (NMIBC) remains to be defined. Immunohistochemical staining and RT-qPCR were performed to screen the expression patterns of Lamin A/C protein and Lamin A mRNA respectively in 58 high and low grade NMIBC specimens. Lamin A/C protein was expressed only in the nucleus and less exhibited in NMIBC tissues compared to non-tumoral ones. On the other side, Lamin A mRNA was up-regulated in NMIBC compared to controls. Nevertheless, both expression patterns (protein and mRNA) were not correlated to clinical prognosis factors and were not able to predict the overall survival of patients with high-grade NMIBC. The deregulation of A-type Lamin is not associated with the prognosis of NMIBC, but it could serve as a diagnostic biomarker distinguishing NMIBC patients from healthy subjects suggesting its involvement as an initiator event of tumorigenesis in our cohort. •Lamin A gene is deregulated in non muscle invasive bladder cancer (NMIBC) tissues both at the mRNA and protein levels.•Lamin A protein and mRNA expression levels’ could significantly discriminate NMIBC patients from healthy controls•Reverse Lamin A mRNa and protein expression’s trends were underscored.•Lamin A protein and mRNA expression’s deregulation were associated to urothelial tumorigenesis’ onset•Deregulated A-type Lamin could serve as an early diagnostic tool for NMIBC