Enhanced pigment removal from intracellular polysaccharides of Arthrospira platensis using macroporous resin NKA-II

Arthrospira platensis intracellular polysaccharides have attracted significant attention due to their wide range of bioactive functions. The presence of residual pigments can negatively impact subsequent separation and purification processes, as well as the development and application of downstream products. In this study, a comparative analysis was conducted to evaluate the decolorization effects of various macroporous resins. The results showed that macroporous resin NKA-II had the highest decolorization rate and retention rate of polysaccharides. To optimize the decolorization process employing NKA-II, a systematic adjustment of various parameters was conducted, leading to the determination of optimal conditions: a sample speed of 3 BV/h (bed volume/h, BV/h), a sample concentration of 6.8 mg/mL, and a sample quantity of 1.3 BV. Under these optimized conditions, three parallel experiments were conducted, and the average comprehensive score was measured as 96.98 ± 1.8 %. Subsequently, a comparison was made between the decolorization effects of NKA-II and those achieved through the use of hydrogen peroxide and activated carbon methods. The findings demonstrated that NKA-II outperformed both hydrogen peroxide and activated carbon in terms of decolorization efficiency. This highlights the superiority of NKA-II as a decolorization material for Arthrospira platensis intracellular polysaccharides. Overall, this study provides valuable insights into the optimization of the Arthrospira platensis intracellular polysaccharide decolorization process and the selection of appropriate decolorization materials, thereby contributing to further research and application of Arthrospira platensis intracellular polysaccharides in various fields. •Macroporous resin adsorption for decolorization of Arthrospira platensis polysaccharide was evaluated and optimized.•Macroporous resin NKA-II had the best effect of decolorization for Arthrospira platensis polysaccharide.•Decolorization process of macroporous resin NKA-II was optimized by response surface method analysis.•Decolorization effect of macroporous resin was better than that of hydrogen peroxide or activated carbon method.