Stable water isotopes reveal modification of cereal water uptake strategies in agricultural co-cropping systems

Agricultural co-cropping is being evaluated in temperate environments as a potential nature-based solution to the changing climate. However, the understanding of underlying physiological processes in co-cropping and its potential to provide climate resilience in temperate agroecosystems remains limited. This study investigated water sources for plants in five distinct cereal-legume co-cropping systems and four of their corresponding cereal monocultures at four main growth stages, under contrasting temperate hydro-climatological conditions in Scotland. Stable water isotope compositions (δ2H and δ18O) for soil water and xylem water were established. Based on the isotope compositions, a Bayesian multi-source mixing model was used to explore proportional soil water uptake patterns for cereal crop plants. Cereals grown in monocultures in this environment took more than 60 % of their water from the upper topsoil (soil depth