Expression profile of RFRP-3 gene in hypothalamic tissue and its relationship with reproductive hormones across phases of the estrous cycle in female rats
The expression profile of RFRP-3 during the different phases of the estrous cycle and its relationship with reproductive hormones were studied in 36 adult female Wister rats. Phases of the estrous cycle were characterized by visual observation of the vagina and vaginal exfoliated cell cytology. The...
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Veröffentlicht in: | Discover Animals 2024-11, Vol.1 (1), Article 34 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The expression profile of RFRP-3 during the different phases of the estrous cycle and its relationship with reproductive hormones were studied in 36 adult female Wister rats. Phases of the estrous cycle were characterized by visual observation of the vagina and vaginal exfoliated cell cytology. The expression profile of the RFRP-3 gene in the hypothalamic tissue was evaluated by real-time qPCR. Reproductive hormones (LH, FSH, estrogen, and progesterone) were measured from plasma by enzyme immunoassay. The expression level of RFRP-3 was significantly higher (P |
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ISSN: | 3004-894X 3004-894X |
DOI: | 10.1007/s44338-024-00037-8 |