Study on the Anti-inflammatory Activity of N-(E)-p-Coumaroyl Tyrosine In Vitro and In Vivo

N -( E )- p -Coumaroyl tyrosine is a phenolic amino acid derivative extracted from the Abrus mollis Hance, Syn of Abrus melanospermus subsp. melanospermus , Fabaceae, which has anti-inflammatory activity. In this study, we aimed to investigate the inflammatory activity and mechanism of N -( E )- p -coumaroyl tyrosine by establishing a lipopolysaccharide-stimulated RAW 264.7 cells in vitro and an injured zebrafish tail fin in vivo . The results showed that N -( E )- p -coumaroyl tyrosine decreased the levels of ROS, NO, IL-6, IL-1β, and IL-18 secreted by LPS-induced RAW 264.7 macrophages. In addition, the results of in vivo studies showed that it inhibited the aggregation of neutrophils and macrophages to the tail after tail cutting stimulation. In summary, N -( E )- p -coumaroyl tyrosine inhibited the production of NO and ROS; decreased the secretions of IL-6, IL-1β, and IL-18; and reduced the aggregation of zebrafish neutrophils and macrophages. Therefore, N -( E )- p -coumaroyl tyrosine may be developed as a potential therapeutic option for inflammatory diseases. Graphical Abstract