Effect of magnetic particles adding into nanostructured hydroxyapatite–alginate composites for orthopedics

The composite materials based on hydroxyapatite (HA), sodium alginate (Alg)–magnetite (Fe 3 O 4 ) were synthesized by the “wet chemistry method” under the influence of microwave irradiation and ultrasound. The biomagnetic samples were investigated by XRD, RFA, SEM, TEM and colorimetric assay methods. The cytotoxicity was assessed on fibroblasts cultures. It was found that the synthesis of Fe 3 O 4 particles in the presence of Alg macromolecules leads to magnetite nanoparticles’ average size decreasing (up to 8 nm). The presence of samples with applied magnetite content (1% of HA) in a nutrient medium did not influence on cells viability. It was shown that the hydrogels were more conducive to cells survival and provide a greater degree of cell proliferation in comparison with beads. It was concluded that Fe 3 O 4 -loaded hydroxyapatite–alginate composites are characterized by good adhesive ability of the fibroblast cells and its bioactivity.