Identification of differentially regulated proteins of Caenorhabditis elegans during Salmonella enterica Serovar Typhi exposure using Mass Spectrometry

Recently, proteomics-based studies have become the primary contributors for the understanding of host–pathogen interactions and the discovery of various drug targets. Herewith, we have used the popular eukaryotic model organism Caenorhabditis elegans to study the host–pathogen interactions at protein levels. Especially, we have employed proteomics approach to monitor the protein players that underwent for differential regulation during host–pathogen interaction through label-free quantitation method using LC–MS/MS technique. As a result of LC–MS/MS analysis, a total of 374 proteins were identified during pathogenic condition when compared to control among which 12 and 04 proteins were upregulated and downregulated with at least 2 folds, respectively. Bioinformatics analyses result suggested that the upregulated proteins have crucial roles in various biological processes including TCA cycle, whereas downregulated proteins were majorly involving in the N-glycosylation synthesis. Additionally, the mRNA levels of up and downregulated proteins were validated using qPCR analysis. Overall, the study suggested that the bacterial infection could target multiple molecular mechanisms to modulate the host machinery which favour the bacterial pathogen for their pathogenesis.