Carbendazim sensitivity of Fusarium solani soil isolates

Four Fusarium solani isolates were isolated from four agricultural soil samples of Murshidabad district, West Bengal, India and identified through morphological characterization and rDNA sequence analysis. Their carbendazim sensitivity were tested through radial growth assay and conidia germination assay. ED 50 value calculated by regressing the mean relative growth values against log concentration of the fungicide was varied from 0.45 to 0.98 ppm. EC 50 value determined by observing 50% inhibition of conidia germination varied from 9.61 to 14.09 ppm. The isolates also showed pleiotropic inhibitory effects on mycelial growth at high (37 °C) and low (10 °C) temperatures in presence of the fungicide. Their tolerance to carbendazim (1 ppm) was reduced in presence of sodium chloride (8%) or sodium dodecyl sulfate (0.05%). The carbendazim tolerance varied among the isolates along with temperature and salt stress.