Porous Ruthenium Selenide Nanoparticle as a Peroxidase Mimic for Glucose Bioassay

Nanozyme is a promising field that offers the substitution for natural enzymes using various nanomaterials. Various nanomaterials with peroxidase-like activity were investigated. Among them, transition metal chalcogenides were explored as promising nanozymes due to their excellent enzyme-mimicking activities. However, ruthenium selenide has not been studied as a peroxidase mimic because of the difficulty for synthesis. Herein, we prepared ruthenium selenide nanomaterial with ordered mesoporous structure (P-RuSe 2 ) employing KIT-6 silica as the template. The composition and structure of P-RuSe 2 were fully characterized. Further, its peroxidase-like activity was investigated. P-RuSe 2 possessed excellent peroxidase-mimicking activity, which catalyzed the oxidation of peroxidase substrates, including 3,3′,5,5′-tetramethylbenzidine, o -phenylenediamine, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in the presence of H 2 O 2 . Moreover, P-RuSe 2 exhibited higher peroxidase-like activity when compared with several representative nanozymes as well as bulk RuSe 2 . To demonstrate its potential applications, the colorimetric detection systems for H 2 O 2 and glucose were successfully constructed based on P-RuSe 2 nanozyme.