Vitamin C Determination in Human Plasma Using an Electro-Activated Pencil Graphite Electrode

The intake of sufficient amounts of vitamin C (ascorbic acid) is necessary for good human health. Therefore, simple, rapid methods for determination of the exact amount of vitamin C in human plasma are important for assessment purposes. A pencil graphite electrode was used to quantitate the amount of vitamin C in human plasma samples. The results indicated that the anodic peak current for ascorbic acid was linear in the concentration range of 10 −6 to 10 −4 M with a correlation coefficient of 0.9998. The obtained detection limit for AA using this amperometry technique was 1.18 × 10 −6 M.