Friss oder stirb! Erweiterung des Substratspektrums von P. putida

The gram-negative bacterium Pseudomonas putida is of increasing interest for industrial applications due to its intrinsic resistance to a broad range of stresses, its metabolic versatility, and the availability of genetic tools. Our group aims to introduce new metabolic pathways by genetic engineering to further expand the metabolic spectrum of this microorganism. Here, we summarize the process of engineering a sucrose consuming strain of P. putida , the obstacles found on the way, and how they were overcome to achieve a stable phenotype.