The Anticancer Efficacy of Platinum Azidothymidin on Hepatocellular Carcinoma Via Affecting the Telomerase and the BcL-2 Genes Expression

Purpose The study of correlation between cancer biomarkers after treatment with anticancer drugs would represent a promising insight into the effectiveness of the drug. Methods In this study, after induction of hepatocellular carcinoma, rats were divided into four groups: groups A and B as healthy o...

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Veröffentlicht in:Journal of gastrointestinal cancer 2020-09, Vol.51 (3), p.813-817
Hauptverfasser: Sabokrouh, Abdolreza, Atabi, Fereshteh, Jassem, Raghed Mohammed, Mohammadi, Reza
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Sprache:eng
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Zusammenfassung:Purpose The study of correlation between cancer biomarkers after treatment with anticancer drugs would represent a promising insight into the effectiveness of the drug. Methods In this study, after induction of hepatocellular carcinoma, rats were divided into four groups: groups A and B as healthy or control group and negative untreated cancer group respectively; groups C and D were treated with platinum azido-thymidine (0.9 mg/kg/day), a novel anti-cancer drug, and azido-thymidine (AZT) (0.3 mg/kg/day) respectively. After induction of cancer, the telomerase and Bcl-2 expression were evaluated by real-time PCR (RT-qPCR), and also Bcl-2 concentration and telomerase activity were measured by enzyme-linked immunosorbent assay (ELISA) and telomerase repeat amplification protocol (TRAP) respectively. Results A significant correlation was observed between telomerase and Bcl-2 in untreated HCC-induced rats as compared to the control group. In untreated cancer group, a direct significant correlation between telomerase activity and expression ( r  = 0.453, p  = 0.022*) and also a negative significant correlation between telomerase activity and Bcl-2 concentration ( r  = − 0.43, p  = 0.034*) and also between telomerase and Bcl-2 expression ( r  = − 0.088, p  = 0.006*) was observed. In drug-treated groups, there was a significant negative correlation between telomerase expression and Bcl-2 concentration ( r  = − 0.45, p  = 0.025) only in the AZT-treated groups. Conclusion Our results indicated a correlation between cancer factors in the untreated cancerous group B and in treated groups only limited to the azithoimidin-treated group (group D). Hence, it may be possible to use this strategy to develop remarkable anticancer drugs in future studies, though this hypothesis requires more in-depth research.
ISSN:1941-6628
1941-6636
DOI:10.1007/s12029-019-00296-9