Effect of bioreactor angle and aeration rate on growth and hydromechanics parameters in bioreactor culture of ginseng suspension cells

Suspension cells of Panax ginseng C.A. Meyer were cultivated in 3-L balloon-type bubble bioreactors and the bioreactor with the angle of 90° at the bottom side was optimized. The gaseous composition in plant cell and tissue cultures is regarded as an important factor affecting the plant growth. Gas hold-up was remarkably higher in the bioreactor with an angle of 90° than the other ones. Aeration rates impacted on the growth ratio, the specific O₂ uptake rate (SOUR) of ginseng cells were investigated. 0.4 vvm was selected as the optimal aeration rate with a dry weight of 6.45 g L⁻¹. The specific O₂ uptake rate in the culture time was detected and reached the top value at the maximum growth ratio.