Anticancer Effect of Total Annonaceous Acetogenins on Hepatocarcinoma

Objective: To confirm the anticancer effect of total annonaceous acetogenins (TAAs) abstracted from Annona squamosa Linn. on human hepatocarcinoma. Methods: The inhibitory effect of TAAs was demonstrated in H,,-bearing mice. The potency of TAAs was confirmed as its 50% inhibiting concentration (IC50...

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Veröffentlicht in:Chinese journal of integrative medicine 2015-09, Vol.21 (9), p.682-688
1. Verfasser: 杨润梅 李雯敏 胡卫军 黄文华 朱春燕 余竞光 赵欣 蔡大勇 高南南
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Sprache:eng
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Zusammenfassung:Objective: To confirm the anticancer effect of total annonaceous acetogenins (TAAs) abstracted from Annona squamosa Linn. on human hepatocarcinoma. Methods: The inhibitory effect of TAAs was demonstrated in H,,-bearing mice. The potency of TAAs was confirmed as its 50% inhibiting concentration (IC50) on Bel-7402 cell under Sulfur Rhodamine B staining. Both underlying mechanisms were explored as cellular apoptosis and cell cycle under flow cytometry. Mitochondrial and recipient apoptotic pathways were differentiated as mitochondrial membrane potential under flow cytometry and caspases activities under fluorescence analysis. Results: The inhibitory rate of TAAs in mice was 50.98% at 4 mg/kg dose. The IC50 of TAAs on Bel-7402 was 20.06 μg/mL (15.13-26.61 μg/mL). Effective mechanisms of TAAs were confirmed as both of arresting cell cycle at G1 phase and inducing apoptosis dose- and time-dependently. Mitochondrial and recipient pathways involved in apoptotic actions of TAAs. Conclusion: TAAs is effective for hepatocarcinoma, via inhibiting proliferation and inducing apoptosis.
ISSN:1672-0415
1993-0402
DOI:10.1007/s11655-014-1845-z