Light-independent accumulation of essential chlorophyll biosynthesis- and photosynthesis-related proteins in Pinus mugo and Pinus sylvestris seedlings

Dark-grown seedlings of Pinus mugo Turra and Pinus sylvestris L. accumulate chlorophyll (Chl) and its precursor protochlorophyllide (Pchlide). Pchlide reduction is a key regulatory step in Chl biosynthesis. In the dark, Pchlide is reduced by light-independent Pchlide oxidoreductase (DPOR) encoded by...

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Veröffentlicht in:Photosynthetica 2010-03, Vol.48 (1), p.16-22
Hauptverfasser: Breznenová, K, Demko, V, Pavlovič, A, Gálová, E, Balážová, R, Hudák, J
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Sprache:eng
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Zusammenfassung:Dark-grown seedlings of Pinus mugo Turra and Pinus sylvestris L. accumulate chlorophyll (Chl) and its precursor protochlorophyllide (Pchlide). Pchlide reduction is a key regulatory step in Chl biosynthesis. In the dark, Pchlide is reduced by light-independent Pchlide oxidoreductase (DPOR) encoded by three plastid genes chlL, chlN, and chlB (chlLNB). To investigate the differences in chlLNB gene expressions, we compared the dark-grown and 24-h illuminated seedlings of P. mugo and P. sylvestris. Expression of these genes was found constitutive in all analyzed samples. We report light-independent accumulation of important proteins involved in Chl biosynthesis (glutamyl-tRNA reductase) and photosystem formation (D1 and LHCI). Chl and Pchlide content and plastid ultrastructure studies were also performed.
ISSN:0300-3604
1573-9058
DOI:10.1007/s11099-010-0004-9