Immobilization of Penicillium solitum lipase on the carbon fiber material “Dnepr-MN”

Immobilization of Penicillium solitum lipase on the carbon fiber material “Dnepr-MN”

A method for immobilizing Penicillium solitum lipase (the medicinal agent Solizyme) on the activated carbon fiber material “Dnepr-MN” is proposed; using a matrix with total pore volumes of 0.41–1.3 cm 3 /g, the method allows the enzyme:matrix ratio (1:30–1:60) to be controlled, yielding preparations...

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Veröffentlicht in:Pharmaceutical chemistry journal 2008-06, Vol.42 (6), p.360-362
Hauptverfasser: Romanovskaya, I. I., Bondarenko, G. I., Davidenko, T. I.
Format: Artikel
Sprache:eng
Schlagworte:
Drug Synthesis Methods and Manufacturing Technology
Medicine
Organic Chemistry
Pharmacology/Toxicology
Pharmacy
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Zusammenfassung:A method for immobilizing Penicillium solitum lipase (the medicinal agent Solizyme) on the activated carbon fiber material “Dnepr-MN” is proposed; using a matrix with total pore volumes of 0.41–1.3 cm 3 /g, the method allows the enzyme:matrix ratio (1:30–1:60) to be controlled, yielding preparations with a specified lipolytic activity (20–40 LU/mg of agent) at quantitative yield (91–97%). Studies of the pH and temperature stability, functioning in acid media, and desorption demonstrated stabilization and prolongation of the lipolytic activity of the immobilized lipase.
ISSN:0091-150X
1573-9031
DOI:10.1007/s11094-008-0127-5