Quantitative luminescent analysis methods

We have developed quantitative luminescent analysis methods not requiring the use of reference media with known contents of the analyte components. The methods are based on relations describing re-absorption of luminescence and also on a relation connecting the luminescence intensity and the absorption coefficients of a multicomponent medium. We present equations allowing us to find the absorption coefficients and consequently the concentrations of the components of the medium from the luminescence intensity measured in relative units. In order to determine the concentrations of nonluminescent components, we also propose and demonstrate the use of a luminescent probe. We present the experimental results for determination of the absorption coefficients and the concentrations of substances by the methods we developed.