Endogenous abscisic acid and protein contents during seed development of Araucaria angustifolia

This paper describes a proteome analysis and changes in endogenous abscisic acid (ABA) contents during seed development of Araucaria angustifolia. Megagametophytes and embryonic axis tissues exhibited a similar ABA variation pattern during seed development, reaching maximum values at the pre-cotyled...

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Veröffentlicht in:Biologia plantarum 2008-03, Vol.52 (1), p.101-104
Hauptverfasser: Silveira, V.,CBB-EUNF, Rio de Janeiro (Brazil). Biotechnology Lab, Santa-Catarina, C.,IB-Univ. of Sao Paulo (Brazil). Dept. of Botany, Balbuena, T.S.,IB-Univ. of Sao Paulo (Brazil). Dept. of Botany, Moraes, F.M.S.,IB-Univ. of Brasilia (Brazil). Dept. of Cell Biology, Ricart, C.A.O.,IB-Univ. of Brasilia (Brazil). Dept. of Cell Biology, Sousa, M.V.,IB-Univ. of Brasilia (Brazil). Dept. of Cell Biology, Guerra, M.P.,CCA-Federal Univ. of Santa Catarina, Florianopolis (Brazil). Dept. of Plant Science, Handro, W.,IB-Univ. of Sao Paulo (Brazil). Dept. of Botany, Floh, E.I.S.,IB-Univ. of Sao Paulo (Brazil). Dept. of Botany
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Sprache:eng
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Zusammenfassung:This paper describes a proteome analysis and changes in endogenous abscisic acid (ABA) contents during seed development of Araucaria angustifolia. Megagametophytes and embryonic axis tissues exhibited a similar ABA variation pattern during seed development, reaching maximum values at the pre-cotyledonary stage. The embryonic axis protein content increased until the cotyledonary stage with following stabilization at mature seed. The two-dimensional electrophoresis at the torpedo developmental stage showed approximately 230 polypeptides against 340 in the mature stage. Peptide mass fingerprinting analyses identified three polypeptides, corresponding to an AtSAC4, a late embryogenesis abundant (LEA) and a storage protein, respectively.
ISSN:0006-3134
1573-8264
1573-8264
DOI:10.1007/s10535-008-0018-3