Actirhodobacter atriluteus gen. nov., sp. nov., isolated from the surface water of the Yellow Sea

A Gram-stain-negative, aerobic, orange-pigmented bacterial strain, designated HHU K3-1 T , was isolated from the surface water of the Yellow Sea. The strain was observed to grow on 2216E agar medium, and growth occurred at pH 6.0–8.0 (optimum 7.0), 28–37 °C (optimum 28 °C), and in the presence of 0.5–5% (w/v) NaCl (optimum 1–3%). The major fatty acids (> 10%) were summed feature 3 (C 16:1 ω 6c/C 16:1 ω 7c), C 17:1 ω 6c and summed feature 8 (C 18:1 ω 6c/C 18:1 ω 7c). Strain HHU K3-1 T was found to contain ubiquinone-10 as the predominant quinone and the major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and sphingoglycolipid (SGL). The 16S rRNA gene sequence analysis indicated that strain HHU K3-1 T shared highest similarities with Pelagerythrobacter marensis KCTC 22370 T (97.7%) and Qipengyuania oceanensis MCCC 1A09965 T (96.9%). However, a phylogenetic tree based on 288 orthologous clusters (OCs) indicated that HHU K3-1 T was close related to Parapontixanthobacter aurantiacus MCCC 1A09962 T . The pairwise AAI and evolutionary distance between HHU K3-1 T and Parapontixanthobacter aurantiacus MCCC 1A09962 T are 67.1% and 0.43, respectively, which meet the recently proposed standard to differentiate genera in the family Erythrobacteraceae . On the basis of the result obtained by the polyphasic taxonomic study, strain HHU K3-1 T can be considered to represent a novel genus in the family Erythrobacteraceae , for which the name Actirhodobacter atriluteus gen. nov., sp. nov. is proposed. The type strain is HHU K3-1 T (= MCCC 1K04225 T = KCTC 72834 T = CGMCC 1.17395 T ).