High-performance thin-layer chromatography chemical fingerprinting: a modern technique for comparative assessment of a multivariate chromatogram analysis of Padina boergesenii

Gujarat’s coastline holds immense storage of valuable marine macroalgae. Padina boergesenii is normally familiar as leafy rolled-blade algae pleasingly studied for its medicinal effects. This alga is therapeutically worthwhile owing to the existence of essential phytoconstituents in it. The purpose of this evaluation was to understand the various steps of methods setup like extraction methods, mobile phase and reagent work on high-performance thin-layer chromatography chemical fingerprinting for marine algae. The qualitative examination of ethanolic and methanolic extracts of algae revealed the presence of a number of bioactive compounds. Toluene‒ethyl acetate (9.3:0.7, V/V ) was employed as the best mobile phase in terms of separation of the band. The pre- and post-derivatizing images at different detection modes confirm the number of bioactive compounds anthraquinone, coumarin, essential oil, saponin, etc. This research concluded that ethanolic and methanolic extract prepared through the Soxhlet method showed a rich source of bioactive compound, which consequently improved its biological activity and this type of activity facilitates numerous pharmaceutical industries.