An accurate high-resolution melting method to genotype bovine β-casein

In the present study, we used high-resolution melting analysis to simultaneously identify the two mutations that taken together give us the genotype of the most common bovine β-casein variants. Allelic variants are detected in real time, and no post-PCR manipulations are required, therefore limiting costs and possible carryover contamination. Data can be copied to a Microsoft Excel spreadsheet for semiautomatic determination of the genotype. The method was validated using different DNA sources, in 122 samples from 12 different bovine populations, obtaining the first report on bovine β-casein genotypes for some of them.