Isolation of Bacillus subtilis: screening for aflatoxins B1, M1, and G1 detoxification

Aflatoxins are commonly found in cereals worldwide and bring significant threats to the food industry and animal production. The aim of this research was to search for Bacillus subtilis from animals’ guts and soil capable of transforming aflatoxin B 1 , M 1 , and G 1 simultaneously. In addition, the selected B. subtilis can inhibit the growth of pathogen and resist to unfavorable conditions within simulated gut environments. High-performance liquid chromatography was used to determine the reduction in aflatoxin concentrations. B. subtilis ANSB060 from fish gut had the strongest ability to detoxify toxins, and percentages of aflatoxin B 1 , M 1 , and G 1 degradation were 81.5%, 60%, and 80.7%, respectively. Moreover, the results implied that the activity of aflatoxins degradation was mainly in the culture supernatant of ANSB060 rather than its cells or cell extracts. And ANSB060 showed antimicrobial activities against Escherichia coli, Salmonella typhimurium , Staphylococcus aureus and resistance to the simulated gut environments. This research indicated that the degradation of aflatoxins by ANSB060 could have a great potential in industrial applications.