Synthesis and anti-tubercular evaluation of some novel pyrazolo[3,4-d]pyrimidine derivatives

A series of phenothiazine clubbed pyrazolo[3,4- d ]pyrimidines have been synthesized by using the Biginelli multi-component cyclocondensation reaction and their ability to inhibit growth of Mycobacterium tuberculosis in vitro have been determined. The results show that compounds 4b , 4d , and 4f exhibited excellent anti-tubercular activity with percentage inhibition of 93, 91, and 96, respectively, at a minimum inhibitory concentration (MIC) of 6.25 μg/ml.