POLYPHENOL OXIDASE IN FUSARIUM OXYSPORUM F. SP. DIANTHI: PARTIAL CHARACTERIZATION AND ACTIVITY

POLYPHENOL OXIDASE IN FUSARIUM OXYSPORUM F. SP. DIANTHI: PARTIAL CHARACTERIZATION AND ACTIVITY

A polyphenol oxidase has been extracted and partially purified from Fusarium oxysporum f. sp. dianthi cultivated in vitro. The enzyme biosynthesis was enhanced by the addition to the medium growth of 3-hydroxyacetophenone. Four different enzyme forms were separated through electrophoresis, and their...

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Veröffentlicht in:Cereal research communications 1997-01, Vol.25 (3), p.835-839
Hauptverfasser: CURIR, P., MERCURI, A., D'AQUILA, F., PASINI, C., DOLCI, M.
Format: Artikel
Sprache:eng
Schlagworte:
Catechols
Enzyme activity
Enzyme substrates
Enzymes
Fusarium
Mycelium
Oral Presentations
Oxidases
Phenols
Plants
Polyphenols
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Zusammenfassung:A polyphenol oxidase has been extracted and partially purified from Fusarium oxysporum f. sp. dianthi cultivated in vitro. The enzyme biosynthesis was enhanced by the addition to the medium growth of 3-hydroxyacetophenone. Four different enzyme forms were separated through electrophoresis, and their apparent molecular mass has been calculated. The enzyme proved to possess a catechol oxidase activity, but not the cresolase one; its oxidative activity has been tested on different substrates.
ISSN:0133-3720
1788-9170
DOI:10.1007/bf03543868