Callus induction and shoot regeneration from stem, rachis and leaf explants in beach pea (Lathyrus japonicus willd)
Combination of naphthaleneacetic acid (NAA) and benzyladenine (BA) affected the callus formation from stem, rachis and leaf explants of beach pea. Mature leaf pieces were best for inducing a callus; when cultured on Murashige and Skoog's (MS) medium supplemented with 4.4 M BA at least 87% of th...
Gespeichert in:
| Veröffentlicht in: | Journal of plant biochemistry and biotechnology 2001-01, Vol.10 (1), p.57-60 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 60 |
|---|---|
| container_issue | 1 |
| container_start_page | 57 |
| container_title | Journal of plant biochemistry and biotechnology |
| container_volume | 10 |
| creator | Debnath, S.C McKenzie, D. B McRae, K.B.(Atlantic Food and Horticulture Research Centre, Kentville (Canada)) |
| description | Combination of naphthaleneacetic acid (NAA) and benzyladenine (BA) affected the callus formation from stem, rachis and leaf explants of beach pea. Mature leaf pieces were best for inducing a callus; when cultured on Murashige and Skoog's (MS) medium supplemented with 4.4 M BA at least 87% of the explants callused regerdless of the NAA concentration. Optimal induction was achieved with 5.4 to 10.7 M NAA depending on the explants used. BA at 4.4 M was always more effective than at the lower concentration of 1.1 M. Multiple buds were induced from calli and formed shoots when transferred to MS medium supplemented with 2.7 M NAA + 4.4 M BA. |
| doi_str_mv | 10.1007/BF03263108 |
| format | Article |
| fullrecord | <record><control><sourceid>fao_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1007_BF03263108</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>IN2001000830</sourcerecordid><originalsourceid>FETCH-LOGICAL-c210t-54aefa87d24672e6d0637aedb7c9a00e02552fa43d1b3a4baa850603efc03f603</originalsourceid><addsrcrecordid>eNpFkM9Lw0AQhRdRsFYv3oU9qhid_ZFsetRitVD0oucw2cy2Kdsk7KZo_3vTKniaj3kf7_AYuxRwLwDMw9MMlMyUgPyIjWBidCKkSY8PLBKTC3HKzmJcA-jUgB6xOEXvt5HXTbW1fd02HJuKx1Xb9jzQkhoKeHi70G547GlzxwPaVR0Poid0nL47j02_L-ElDSHvCPn1AvvVLgzda-zaprYDfdXeVzfn7MShj3Txd8fsc_b8MX1NFu8v8-njIrFSQJ-kGslhbiqpMyMpqyBTBqkqjZ0gAIFMU-lQq0qUCnWJmKeQgSJnQbkBxuz2t9eGNsZAruhCvcGwKwQU-7mK_7kG-epXdtgWuAx1LOZvEmDwhhTUD8C7Zrw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Callus induction and shoot regeneration from stem, rachis and leaf explants in beach pea (Lathyrus japonicus willd)</title><source>Springer Nature - Complete Springer Journals</source><creator>Debnath, S.C ; McKenzie, D. B ; McRae, K.B.(Atlantic Food and Horticulture Research Centre, Kentville (Canada))</creator><creatorcontrib>Debnath, S.C ; McKenzie, D. B ; McRae, K.B.(Atlantic Food and Horticulture Research Centre, Kentville (Canada))</creatorcontrib><description>Combination of naphthaleneacetic acid (NAA) and benzyladenine (BA) affected the callus formation from stem, rachis and leaf explants of beach pea. Mature leaf pieces were best for inducing a callus; when cultured on Murashige and Skoog's (MS) medium supplemented with 4.4 M BA at least 87% of the explants callused regerdless of the NAA concentration. Optimal induction was achieved with 5.4 to 10.7 M NAA depending on the explants used. BA at 4.4 M was always more effective than at the lower concentration of 1.1 M. Multiple buds were induced from calli and formed shoots when transferred to MS medium supplemented with 2.7 M NAA + 4.4 M BA.</description><identifier>ISSN: 0971-7811</identifier><identifier>EISSN: 0974-1275</identifier><identifier>DOI: 10.1007/BF03263108</identifier><language>eng</language><subject>CAL ; CALLO ; CALLUS ; CULTIVO DE TEJIDOS ; CULTURE DE TISSU ; LATHYRUS ; PLANT GROWTH SUBSTANCES ; SUBSTANCE DE CROISSANCE VEGETALE ; SUSTANCIAS DE CRECIMIENTO VEGETAL ; TISSUE CULTURE</subject><ispartof>Journal of plant biochemistry and biotechnology, 2001-01, Vol.10 (1), p.57-60</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c210t-54aefa87d24672e6d0637aedb7c9a00e02552fa43d1b3a4baa850603efc03f603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Debnath, S.C</creatorcontrib><creatorcontrib>McKenzie, D. B</creatorcontrib><creatorcontrib>McRae, K.B.(Atlantic Food and Horticulture Research Centre, Kentville (Canada))</creatorcontrib><title>Callus induction and shoot regeneration from stem, rachis and leaf explants in beach pea (Lathyrus japonicus willd)</title><title>Journal of plant biochemistry and biotechnology</title><description>Combination of naphthaleneacetic acid (NAA) and benzyladenine (BA) affected the callus formation from stem, rachis and leaf explants of beach pea. Mature leaf pieces were best for inducing a callus; when cultured on Murashige and Skoog's (MS) medium supplemented with 4.4 M BA at least 87% of the explants callused regerdless of the NAA concentration. Optimal induction was achieved with 5.4 to 10.7 M NAA depending on the explants used. BA at 4.4 M was always more effective than at the lower concentration of 1.1 M. Multiple buds were induced from calli and formed shoots when transferred to MS medium supplemented with 2.7 M NAA + 4.4 M BA.</description><subject>CAL</subject><subject>CALLO</subject><subject>CALLUS</subject><subject>CULTIVO DE TEJIDOS</subject><subject>CULTURE DE TISSU</subject><subject>LATHYRUS</subject><subject>PLANT GROWTH SUBSTANCES</subject><subject>SUBSTANCE DE CROISSANCE VEGETALE</subject><subject>SUSTANCIAS DE CRECIMIENTO VEGETAL</subject><subject>TISSUE CULTURE</subject><issn>0971-7811</issn><issn>0974-1275</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNpFkM9Lw0AQhRdRsFYv3oU9qhid_ZFsetRitVD0oucw2cy2Kdsk7KZo_3vTKniaj3kf7_AYuxRwLwDMw9MMlMyUgPyIjWBidCKkSY8PLBKTC3HKzmJcA-jUgB6xOEXvt5HXTbW1fd02HJuKx1Xb9jzQkhoKeHi70G547GlzxwPaVR0Poid0nL47j02_L-ElDSHvCPn1AvvVLgzda-zaprYDfdXeVzfn7MShj3Txd8fsc_b8MX1NFu8v8-njIrFSQJ-kGslhbiqpMyMpqyBTBqkqjZ0gAIFMU-lQq0qUCnWJmKeQgSJnQbkBxuz2t9eGNsZAruhCvcGwKwQU-7mK_7kG-epXdtgWuAx1LOZvEmDwhhTUD8C7Zrw</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Debnath, S.C</creator><creator>McKenzie, D. B</creator><creator>McRae, K.B.(Atlantic Food and Horticulture Research Centre, Kentville (Canada))</creator><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200101</creationdate><title>Callus induction and shoot regeneration from stem, rachis and leaf explants in beach pea (Lathyrus japonicus willd)</title><author>Debnath, S.C ; McKenzie, D. B ; McRae, K.B.(Atlantic Food and Horticulture Research Centre, Kentville (Canada))</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c210t-54aefa87d24672e6d0637aedb7c9a00e02552fa43d1b3a4baa850603efc03f603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>CAL</topic><topic>CALLO</topic><topic>CALLUS</topic><topic>CULTIVO DE TEJIDOS</topic><topic>CULTURE DE TISSU</topic><topic>LATHYRUS</topic><topic>PLANT GROWTH SUBSTANCES</topic><topic>SUBSTANCE DE CROISSANCE VEGETALE</topic><topic>SUSTANCIAS DE CRECIMIENTO VEGETAL</topic><topic>TISSUE CULTURE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Debnath, S.C</creatorcontrib><creatorcontrib>McKenzie, D. B</creatorcontrib><creatorcontrib>McRae, K.B.(Atlantic Food and Horticulture Research Centre, Kentville (Canada))</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Journal of plant biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Debnath, S.C</au><au>McKenzie, D. B</au><au>McRae, K.B.(Atlantic Food and Horticulture Research Centre, Kentville (Canada))</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Callus induction and shoot regeneration from stem, rachis and leaf explants in beach pea (Lathyrus japonicus willd)</atitle><jtitle>Journal of plant biochemistry and biotechnology</jtitle><date>2001-01</date><risdate>2001</risdate><volume>10</volume><issue>1</issue><spage>57</spage><epage>60</epage><pages>57-60</pages><issn>0971-7811</issn><eissn>0974-1275</eissn><abstract>Combination of naphthaleneacetic acid (NAA) and benzyladenine (BA) affected the callus formation from stem, rachis and leaf explants of beach pea. Mature leaf pieces were best for inducing a callus; when cultured on Murashige and Skoog's (MS) medium supplemented with 4.4 M BA at least 87% of the explants callused regerdless of the NAA concentration. Optimal induction was achieved with 5.4 to 10.7 M NAA depending on the explants used. BA at 4.4 M was always more effective than at the lower concentration of 1.1 M. Multiple buds were induced from calli and formed shoots when transferred to MS medium supplemented with 2.7 M NAA + 4.4 M BA.</abstract><doi>10.1007/BF03263108</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0971-7811 |
| ispartof | Journal of plant biochemistry and biotechnology, 2001-01, Vol.10 (1), p.57-60 |
| issn | 0971-7811 0974-1275 |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_BF03263108 |
| source | Springer Nature - Complete Springer Journals |
| subjects | CAL CALLO CALLUS CULTIVO DE TEJIDOS CULTURE DE TISSU LATHYRUS PLANT GROWTH SUBSTANCES SUBSTANCE DE CROISSANCE VEGETALE SUSTANCIAS DE CRECIMIENTO VEGETAL TISSUE CULTURE |
| title | Callus induction and shoot regeneration from stem, rachis and leaf explants in beach pea (Lathyrus japonicus willd) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T03%3A19%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-fao_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Callus%20induction%20and%20shoot%20regeneration%20from%20stem,%20rachis%20and%20leaf%20explants%20in%20beach%20pea%20(Lathyrus%20japonicus%20willd)&rft.jtitle=Journal%20of%20plant%20biochemistry%20and%20biotechnology&rft.au=Debnath,%20S.C&rft.date=2001-01&rft.volume=10&rft.issue=1&rft.spage=57&rft.epage=60&rft.pages=57-60&rft.issn=0971-7811&rft.eissn=0974-1275&rft_id=info:doi/10.1007/BF03263108&rft_dat=%3Cfao_cross%3EIN2001000830%3C/fao_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |