Callus induction and shoot regeneration from stem, rachis and leaf explants in beach pea (Lathyrus japonicus willd)

Combination of naphthaleneacetic acid (NAA) and benzyladenine (BA) affected the callus formation from stem, rachis and leaf explants of beach pea. Mature leaf pieces were best for inducing a callus; when cultured on Murashige and Skoog's (MS) medium supplemented with 4.4 M BA at least 87% of the explants callused regerdless of the NAA concentration. Optimal induction was achieved with 5.4 to 10.7 M NAA depending on the explants used. BA at 4.4 M was always more effective than at the lower concentration of 1.1 M. Multiple buds were induced from calli and formed shoots when transferred to MS medium supplemented with 2.7 M NAA + 4.4 M BA.