Sunflower Nitrate Reductase: Purification and Characterization

Sunflower Nitrate Reductase: Purification and Characterization

A single isoform, NADH: nitrate reductase (NR), was purified 500 folds from sunflower leaves by affinity chromatography on Blue Sepharose CL-6B. Purified NR had a pH optima of 7.25 and a molecular weight of 210 kD. In SDS-PAGE, two bands of 47 and 56 kD were obtained. NADH: ferric citrate reductase...

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Veröffentlicht in:Journal of plant biochemistry and biotechnology 1999, Vol.8 (1), p.41-45
Hauptverfasser: Pattanayak, Debasis, Chatterjee, Sukumar R
Format: Artikel
Sprache:eng
Schlagworte:
affinity chromatography
agarose
Helianthus annuus
leaves
molecular weight
NAD (coenzyme)
nitrate reductase
polyacrylamide gel electrophoresis
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Zusammenfassung:A single isoform, NADH: nitrate reductase (NR), was purified 500 folds from sunflower leaves by affinity chromatography on Blue Sepharose CL-6B. Purified NR had a pH optima of 7.25 and a molecular weight of 210 kD. In SDS-PAGE, two bands of 47 and 56 kD were obtained. NADH: ferric citrate reductase activity was copurified with NR with a specific activity of 2. The Vmax of NADH: ferric citrate reductase was 8.69 units mg⁻¹ protein and the apparent Km for ferric citrate was 0.435 mM.
ISSN:0971-7811
0974-1275
DOI:10.1007/BF03263056