Direct electrochemistry of hemoglobin in a nafion and CuS microsphere modified carbon ionic liquid electrode and its electrocatalytic behavior
Direct electrochemistry of hemoglobin (Hb) was realized on a Nafion and CuS microsphere composite film modified carbon ionic liquid electrode (CILE) with N -butylpyridinium hexafluorophosphate (BPPF6) as binder. Scanning electron microscopy (SEM), UV-Vis absorption spectroscopy and cyclic voltammetr...
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Veröffentlicht in: | Journal of the Iranian Chemical Society 2010-06, Vol.7 (2), p.470-477 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Direct electrochemistry of hemoglobin (Hb) was realized on a Nafion and CuS microsphere composite film modified carbon ionic liquid electrode (CILE) with
N
-butylpyridinium hexafluorophosphate (BPPF6) as binder. Scanning electron microscopy (SEM), UV-Vis absorption spectroscopy and cyclic voltammetry were used to characterize the fabricated Nafion/CuS/Hb/CILE. Experimental results showed that a pair of well-defined quasi-reversible redox peaks appeared with the formal potential as −0.386 V (
vs
. SCE) in pH 7.0 Britton-Robinson (B-R) buffer solution, which was attributed to the Hb heme Fe(III)/Fe(II) redox couples. The electrochemical parameters of Hb in the composite film were carefully investigated with the charge transfer coefficient (α), the electron transfer number (n) and the electron transfer rate constant (
k
s
) as 0.505, 1.196 and 0.610 s
−1
, respectively. The composite film provided a favorable microenvironment for retaining the native structure of Hb. The presence of CuS microspheres showed great improvement on the electron transfer rate of Hb with the CILE, which maybe due to the contribution of specific characteristics of CuS microspheres and the inherent advantages of ionic liquid on the modified electrode. The fabricated Hb modified electrode showed good electrocatalytic ability in the reduction of H
2
O
2
. The proposed bioelectrode can be used as a new third generation H
2
O
2
biosensor. |
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ISSN: | 1735-207X 1735-2428 |
DOI: | 10.1007/BF03246034 |