Evaluation of antioxidant activity. I. Application of an enzyme-catalyzed system [Fatty acids]

A study was made of the inhibitory effect of the antioxidants propyl gallate, tertiary butylated hydroxyanisol and ethoxyquin on the enzyme‐ catalyzed oxygenation of linolenic acid. Gas chro‐matography was used to measure unchanged fatty acid after varying incubation periods and at various concentrations of antioxidant. Propyl gallate was also tested for its influence on the oxygenation of an emulsion of linoleic acid. The effect of the antioxidants is compared with results obtained previously with a linoleic acid emulsion. It is concluded that it is advantageous to apply linoleic acid as substrate in the model system.