Abnormal Growth of Habituated Sugarbeet Callus and Cell Suspensions

Habituated sugarbeet callus and cell suspension cultures derived therefrom, compared to hormone-dependent normal cultures, exhibit a shorter linear growth phase, although they divide actively. Microscopic observations indicate deficiencies in cell expansion and absence of cell differentiation. Cell...

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Veröffentlicht in:In vitro cellular & developmental biology. Plant 1991-07, Vol.27P (3), p.112-116
Hauptverfasser: Daniel Hagege, Claire Kevers, Gaspar, Thomas, Thorpe, Trevor A.
Format: Artikel
Sprache:eng
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Zusammenfassung:Habituated sugarbeet callus and cell suspension cultures derived therefrom, compared to hormone-dependent normal cultures, exhibit a shorter linear growth phase, although they divide actively. Microscopic observations indicate deficiencies in cell expansion and absence of cell differentiation. Cell expansion apparently is interrupted by a cell "budding" process. Some of the cells seem to be empty due to ballooning out of the protoplasm and the bursting of the cell membrane by defective cell wall development. A low amount of cellulose was confirmed by microspectrophotometric estimation. Such cultures exhibit all the characteristics of vitrified tissue.
ISSN:1054-5476
1475-2689
DOI:10.1007/BF02632193