Normal Murine Melanocytes in Culture

A major obstacle to applying the techniques of molecular biology to the genetics and cell biology of pigmentation has been our inability to grow normal murine melanocytes in culture. We report here the establishment and characterization of continuously proliferating cultures of cutaneous pigment cel...

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Veröffentlicht in:	In Vitro Cellular & Developmental Biology 1987-07, Vol.23 (7), p.519-522
Hauptverfasser:	Tamura, Akihiko, Halaban, Ruth, Moellmann, Gisela, Cowan, Janet M., Lerner, Michael R., Lerner, Aaron B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Animals Animals, Newborn Biological and medical sciences Biotechnology Cell cultures. Hybridization. Fusion Cell Division - drug effects Cell growth Cells, Cultured Chromosomes Culture Techniques - methods Cultured cells Dermis DNA Replication - drug effects Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fibroblasts Fundamental and applied biological sciences. Psychology Human genetics Human growth Karyotyping Kinetics Melanocytes Melanocytes - cytology Methods. Procedures. Technologies Mice Mice, Inbred Strains Molecular and cellular biology Placental extracts Rapid Communications in Cell Biology Species Specificity Tetradecanoylphorbol Acetate - pharmacology
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Zusammenfassung:	A major obstacle to applying the techniques of molecular biology to the genetics and cell biology of pigmentation has been our inability to grow normal murine melanocytes in culture. We report here the establishment and characterization of continuously proliferating cultures of cutaneous pigment cells from seven strains of mice. Melanocytes were grown from the dermis of newborn mice in medium containing 12-0-tetradecanoyl-13-phorbol-acetate; a substance, such as melanotropin, that raises intracellular levels of cyclic AMP; and an extract made from human placenta.
ISSN:	0883-8364 2327-431X 1475-2689
DOI:	10.1007/BF02628423