Establishment and Characterization of a Cell Line (SS78) from a Human Renal Cell Carcinoma
A new cell line, SS78, was established from a primary renal cell carcinoma of a Caucasian male. The tissue was dispersed with collagenase, and viable cells were separated by flotation on a Ficoll-Hypaque gradient. In culture, the SS78 cells retained a distinct epithelial morphology, and no fibroblas...
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Veröffentlicht in: | In Vitro 1981-12, Vol.17 (12), p.1117-1124 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A new cell line, SS78, was established from a primary renal cell carcinoma of a Caucasian male. The tissue was dispersed with collagenase, and viable cells were separated by flotation on a Ficoll-Hypaque gradient. In culture, the SS78 cells retained a distinct epithelial morphology, and no fibroblastlike cells were seen. The cultured cells were aneuploid with a modal chromosome number of 80 and had several marker chromosomes. Inoculation of the cultured cells into athymic nude mice caused tumors at the sites of inoculation. |
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ISSN: | 0073-5655 1475-2689 |
DOI: | 10.1007/BF02618614 |