The effects of ototoxic drugs on mechano-electric transduction channels in chick cochlear hair cells

The effects of ototoxic drugs on mechano-electrical transduction (MET) currents were investigated in dissociated cochlear hair cells of the chick, using a whole-cell patch-electrode voltage-clamp technique. Dihydrostreptomycin (DHSM) and cisplatin (cis-dichlorodiammine platinum II, CDDP) blocked the...

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Veröffentlicht in:	European archives of oto-rhino-laryngology 1994-08, Vol.251 Suppl 1 (S1), p.S53-S56
Hauptverfasser:	Kimitsuki, T, Nakagawa, T, Hisashi, K, Komune, S, Uemura, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anti-Bacterial Agents - adverse effects Anti-Bacterial Agents - pharmacokinetics Antineoplastic Agents - adverse effects Antineoplastic Agents - pharmacokinetics Chickens Cisplatin - adverse effects Cisplatin - pharmacokinetics Cochlea - drug effects Cochlea - metabolism Dihydrostreptomycin Sulfate - adverse effects Dihydrostreptomycin Sulfate - pharmacokinetics Dose-Response Relationship, Drug Hair Cells, Auditory - drug effects Hair Cells, Auditory - metabolism Patch-Clamp Techniques - methods Signal Transduction - drug effects
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Zusammenfassung:	The effects of ototoxic drugs on mechano-electrical transduction (MET) currents were investigated in dissociated cochlear hair cells of the chick, using a whole-cell patch-electrode voltage-clamp technique. Dihydrostreptomycin (DHSM) and cisplatin (cis-dichlorodiammine platinum II, CDDP) blocked the MET channel in a dose- and voltage-dependent manner. In contrast, acetyl salicylate did not suppress the MET current. At -50 mV, DHSM and CDDP blocked the MET channel with a Hill coefficient of 0.93 and 2.1, respectively. These findings suggest that a single DHSM molecule or more than one CDDP molecule binds to a single MET channel.
ISSN:	0937-4477 1434-4726
DOI:	10.1007/BF02565220