Transesterification of oil by fatty acid‐modified lipase

Fatty acid was covalently attached to lipase (EC 3.1.1.3.) fromPhycomyces nites, yielding a modified lipase of higher specific activity in hydrolytic and synthetic reactions in organic solvents. Attached long‐chain fatty acids solubilized the lipase in organic solvent and, therefore, promotion of di...

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Veröffentlicht in:	Journal of the American Oil Chemists' Society 1993-06, Vol.70 (6), p.571-574
Hauptverfasser:	Murakami, Mototake, Kawasaki, Yoshihiro, Kawanari, Masami, Okai, Hideo
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Dispersibility Fat industries fatty acid‐modified lipase Food industries Fundamental and applied biological sciences. Psychology organic solvent selectivity transesterification
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Beschreibung
Zusammenfassung:	Fatty acid was covalently attached to lipase (EC 3.1.1.3.) fromPhycomyces nites, yielding a modified lipase of higher specific activity in hydrolytic and synthetic reactions in organic solvents. Attached long‐chain fatty acids solubilized the lipase in organic solvent and, therefore, promotion of dispersibility in organic solvent resulted in much higher reactivity. The initial rate of transesterification by modified lipase was almost 40 times that of native lipase in organic solvent. The specificities and selectivity of the modified lipase depended on the kind of attached fatty acid.
ISSN:	0003-021X 1558-9331
DOI:	10.1007/BF02545321