Monoglyceride analysis with reversed phase HPLC

A simple, convenient method for the simultaneous determination of the homologous distribution and ratio of positional isomers of monoglycerides in commercial products has been developed. Monoglycerides were analyzed with reversed phase HPLC using the glyceride‐selective post‐column reactor detector (GS‐PCRD). The GS‐PCRD indicated molar responsibility to all monoglycerides up to monostearin. It can, for example, detect 0.07 nmol of 1‐monolaurin and give a linear working curve between 0.18–80.3 nmol of 1‐monolaurin. Furthermore, monoglycerides in commercial margarines could be selectively detected and quantitatively determined without any pretreatment. Therefore, the proposed method should be applicable to the analysis of monoglycerides in various commercial products.