Separation of cholesterol, and fatty acylglycerols, acids and amides by thin-layer chromatography

A rapid unidimensional thin‐layer chromatographic (TLC) method for the separation of neutral lipids is described, using two sequential solvent systems of different polarity. Excellent separations of mono‐, di‐ and triglycerides, fatty acids, fatty amides, and cholesterol are thereby achieved. Separa...

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Veröffentlicht in:	Lipids 1991-05, Vol.26 (5), p.405-406
Hauptverfasser:	Bilyk, A, Piazza, G.J, Bistline, R.G. Jr, Haas, M.J
Format:	Artikel
Sprache:	eng
Schlagworte:	acylglycerols amides Analytical, structural and metabolic biochemistry Biological and medical sciences cholesterol fatty acids Fundamental and applied biological sciences. Psychology Lipids Miscellaneous Other biological molecules rapid methods separation thin layer chromatography
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Beschreibung
Zusammenfassung:	A rapid unidimensional thin‐layer chromatographic (TLC) method for the separation of neutral lipids is described, using two sequential solvent systems of different polarity. Excellent separations of mono‐, di‐ and triglycerides, fatty acids, fatty amides, and cholesterol are thereby achieved. Separation is accomplished at room temperature and requires 25 min.
ISSN:	0024-4201 1558-9307
DOI:	10.1007/BF02537208