A PCR-based method for mating type determination in Cochliobolus heterostrophus

A PCR-based method for mating type determination in Cochliobolus heterostrophus

A rapid and accurate method based on multiplex PCR using three different primers was developed for determining mating type in Cochliobolus heterostrophus. The primers of MAT113 and MAT123 were uniquely derived from MAT1-1 and MAT1-2, respectively, and the primer MATcon5 is conserved in the flanking...

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Veröffentlicht in:Mycoscience 1997-12, Vol.38 (4), p.455-458
Hauptverfasser: Gafur, Abdul, Tanaka, Chihiro, Ouchi, Seiji, Tsuda, Mitsuya
Format: Artikel
Sprache:eng
Schlagworte:
BIOLOGICAL DIFFERENCES
COCHLIOBOLUS HETEROSTROPHUS
DIFERENCIAS BIOLOGICAS
DIFFERENCE BIOLOGIQUE
mating type
PCR
polymerase chain reaction (PCR)
ZEA MAYS
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Beschreibung
Zusammenfassung:A rapid and accurate method based on multiplex PCR using three different primers was developed for determining mating type in Cochliobolus heterostrophus. The primers of MAT113 and MAT123 were uniquely derived from MAT1-1 and MAT1-2, respectively, and the primer MATcon5 is conserved in the flanking regions of the idiomorphso The amplification product was estimated to be 702 bp (MAT1-1) or 547 bp (MAT1-2). Crossing experiments confirmed the accuracy of this method, which requires less time than the conventional determination of mating type.
ISSN:1340-3540
1618-2545
DOI:10.1007/BF02461689