Purification and some properties of an isoform of metal proteinases from Hypsizygus marmoreus grown on sawdust culture

Purification and some properties of an isoform of metal proteinases from Hypsizygus marmoreus grown on sawdust culture

Purification and some properties of an isoform of metal proteinase from Hypsizygus marmoreus are described. This enzyme was purified 711-fold with 5.44% recovery. The molecular weight and pl value were 41,500 and pH 7.7, respectively. The highest activity was observed against milk casein as the subs...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Mycoscience 1998-12, Vol.39 (4), p.471-474
Hauptverfasser: Terashita, Takao, Nakaie, Yoko, Yoshikawa, Kentaro, Shishiyama, Jiko
Format: Artikel
Sprache:eng
Schlagworte:
AGARICALES
CHAMPIGNON COMESTIBLE
EDIBLE FUNGI
fruit-body formation
Hypsizygus marmoreus
INHIBIDORES DE PROTEINASAS
INHIBITEUR DE PROTEINASES
metal proteinase
mushroom
PROTEASAS
PROTEASE
PROTEASES
proteinase inhibitor
PROTEINASE INHIBITORS
SETAS COMESTIBLES
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Purification and some properties of an isoform of metal proteinase from Hypsizygus marmoreus are described. This enzyme was purified 711-fold with 5.44% recovery. The molecular weight and pl value were 41,500 and pH 7.7, respectively. The highest activity was observed against milk casein as the substrate. This enzyme was strongly inhibited by metal proteinase inhibitors such as phosphoramidon, EDTA, and o-phenanthroline.
ISSN:1340-3540
1618-2545
DOI:10.1007/BF02460908