On the role of divalent cations in the reaction mechanism of malic enzyme

On the role of divalent cations in the reaction mechanism of malic enzyme

The kinetic order of addition of Mg2+ and L-malate to malic enzyme has been determined. Mg2+ is the first to bind, and probably acts as a link between the substrate and the active site.

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Veröffentlicht in:Experientia 1975-10, Vol.31 (10), p.1126-1128
Hauptverfasser: Massarini, E, Cazzulo, J J
Format: Artikel
Sprache:eng
Schlagworte:
Cations, Divalent
Cobalt - pharmacology
Kinetics
Magnesium - pharmacology
Malate Dehydrogenase - metabolism
Manganese - pharmacology
Pseudomonas - enzymology
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Zusammenfassung:The kinetic order of addition of Mg2+ and L-malate to malic enzyme has been determined. Mg2+ is the first to bind, and probably acts as a link between the substrate and the active site.
ISSN:0014-4754
1420-9071
DOI:10.1007/BF02326748