PKC activators stimulate H+ conductance in chicken enterocytes

Chicken enterocytes present a H+-conducting pathway involved in the recovery of intracellular pH (pHi) from an acid load. In the current study we have tested the effect of protein kinase C (PKC) activators on the rate of proton efflux through the H+-conducting pathway. The rate of proton efflux was increased by the addition of 1,2-dioctanoyl-rac-glycerol (DOG) or phorbol 12-myristate 13-acetate (PMA), but it was not affected by the addition of the inactive phorbol ester analogue, 4alpha-phorbol 12, 13-didecanoate. DOG stimulated the process in a dose-dependent manner with a half-maximal effect at 45 microM. Staurosporine and Zn2+ prevented the DOG-dependent increase in the rate of proton efflux. The rate of proton efflux was affected by the pH, and DOG shifted this relationship upward and to the right. These results suggest that the proton-conducting pathway is regulated by PKC.