Preparative electrophoresis for large scale preparation of highly purified bovine growth hormone
A method allowing large scale preparation of bovine growth hormone from pituitary glands in relatively few steps is described. In comparison to conventional purification techniques previously reported, the use of preparative polyacrylamide gel electrophoresis reduces the number of steps and the amou...
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Veröffentlicht in: | Experientia 1983-01, Vol.39 (9), p.1052-1055 |
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Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
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Online-Zugang: | Volltext |
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Zusammenfassung: | A method allowing large scale preparation of bovine growth hormone from pituitary glands in relatively few steps is described. In comparison to conventional purification techniques previously reported, the use of preparative polyacrylamide gel electrophoresis reduces the number of steps and the amount of time needed for the isolation of the hormone. In addition, the yield several times greater, the hormone is pure and it has greater bioactivity (1.83 IU/mg). |
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ISSN: | 0014-4754 1420-9071 |
DOI: | 10.1007/BF01989802 |