Fetal rat brain hemisphere tissue in nonadherent stationary organ culture
A simple organ culture system for brain tissue is described. Fragments of fetal rat brain hemisphere tissue are explanted to multiwell dishes base-coated with semisolid agar. In this system nonadherent organ culture can be performed for at least 50 days. Cell migration, biochemical and morphological...
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| Veröffentlicht in: | Experientia 1985-12, Vol.41 (12), p.1517-1524 |
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| container_title | Experientia |
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| creator | STEINSVAG, S. K LAERUM, O. D |
| description | A simple organ culture system for brain tissue is described. Fragments of fetal rat brain hemisphere tissue are explanted to multiwell dishes base-coated with semisolid agar. In this system nonadherent organ culture can be performed for at least 50 days. Cell migration, biochemical and morphological differentiation and the formation of a layered architecture seem to mimic some of the phenomena occurring in the developing rat brain in vivo. The fragments may therefore be a useful organ culture model for nervous tissue. |
| doi_str_mv | 10.1007/BF01964788 |
| format | Article |
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The fragments may therefore be a useful organ culture model for nervous tissue.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - cytology</subject><subject>Brain - embryology</subject><subject>Brain - ultrastructure</subject><subject>Cell Differentiation</subject><subject>Cell Survival</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Experimental organogenesis</subject><subject>Fetus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glial Fibrillary Acidic Protein - analysis</subject><subject>Immunoenzyme Techniques</subject><subject>Microscopy, Electron</subject><subject>Morphogenesis</subject><subject>Organ Culture Techniques - methods</subject><subject>Organogenesis. 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| ispartof | Experientia, 1985-12, Vol.41 (12), p.1517-1524 |
| issn | 0014-4754 1420-9071 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Biological and medical sciences Brain - cytology Brain - embryology Brain - ultrastructure Cell Differentiation Cell Survival Embryology: invertebrates and vertebrates. Teratology Experimental organogenesis Fetus Fundamental and applied biological sciences. Psychology Glial Fibrillary Acidic Protein - analysis Immunoenzyme Techniques Microscopy, Electron Morphogenesis Organ Culture Techniques - methods Organogenesis. Physiological fonctions Phosphopyruvate Hydratase - analysis Rats Time Factors |
| title | Fetal rat brain hemisphere tissue in nonadherent stationary organ culture |
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