Fetal rat brain hemisphere tissue in nonadherent stationary organ culture

A simple organ culture system for brain tissue is described. Fragments of fetal rat brain hemisphere tissue are explanted to multiwell dishes base-coated with semisolid agar. In this system nonadherent organ culture can be performed for at least 50 days. Cell migration, biochemical and morphological...

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Veröffentlicht in:	Experientia 1985-12, Vol.41 (12), p.1517-1524
Hauptverfasser:	STEINSVAG, S. K, LAERUM, O. D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Brain - cytology Brain - embryology Brain - ultrastructure Cell Differentiation Cell Survival Embryology: invertebrates and vertebrates. Teratology Experimental organogenesis Fetus Fundamental and applied biological sciences. Psychology Glial Fibrillary Acidic Protein - analysis Immunoenzyme Techniques Microscopy, Electron Morphogenesis Organ Culture Techniques - methods Organogenesis. Physiological fonctions Phosphopyruvate Hydratase - analysis Rats Time Factors
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Beschreibung
Zusammenfassung:	A simple organ culture system for brain tissue is described. Fragments of fetal rat brain hemisphere tissue are explanted to multiwell dishes base-coated with semisolid agar. In this system nonadherent organ culture can be performed for at least 50 days. Cell migration, biochemical and morphological differentiation and the formation of a layered architecture seem to mimic some of the phenomena occurring in the developing rat brain in vivo. The fragments may therefore be a useful organ culture model for nervous tissue.
ISSN:	0014-4754 1420-9071
DOI:	10.1007/BF01964788