A source of error in the fluorometric determination of protein in human placental microsomes

A source of error in the fluorometric determination of protein in human placental microsomes

When protein is assayed in human placental microsomes by the fluorescamine procedure, 25--30% of the observed fluorescence results from labeling of phospholipids.

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Bibliographische Detailangaben
Veröffentlicht in:Experientia 1979-05, Vol.35 (5), p.707-707
Hauptverfasser: Blomquist, C H, Kotts, C E
Format: Artikel
Sprache:eng
Schlagworte:
Female
Humans
In Vitro Techniques
Membrane Lipids - analysis
Methods
Microsomes - analysis
Phospholipids - analysis
Placenta - analysis
Placenta - ultrastructure
Pregnancy
Proteins - analysis
Spectrometry, Fluorescence
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Beschreibung
Zusammenfassung:When protein is assayed in human placental microsomes by the fluorescamine procedure, 25--30% of the observed fluorescence results from labeling of phospholipids.
ISSN:0014-4754
1420-9071
DOI:10.1007/BF01960411