Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry

Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-act...

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Veröffentlicht in:	Experientia 1989-02, Vol.45 (2), p.186-190
Hauptverfasser:	OLTZ, E. M, POLLACK, S, DELOHERY, T, SMITH, M. J, OJIKA, M, LEE, S, KUSTIN, K, NAKANISHI, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Ascidia ceratodes Ascidia nigra Biological and medical sciences Blood Cells - analysis Cell Separation Flow Cytometry Fundamental and applied biological sciences. Psychology Invertebrates Less representative or incertae sedis groups: acanthocephala, chaetognatha, gnathostomulida, lophophoria, merostomata, mesozoa, myxozoa, nematorhyncha, pararthropoda, placozoa, priapuloidea, prochordata, pycnogonida, rotifera Marine Organic Chemicals Pigments, Biological - analysis Urochordata - analysis Vanadium - analysis
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Zusammenfassung:	Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) and chemically analyzed. V was found in the spherical, green/grey signet ring cells, and to a lesser degree in the mulberry-shaped, yellow/green morula cells (MRs), whereas free TC was detected mainly in MRs.
ISSN:	0014-4754 1420-9071
DOI:	10.1007/BF01954871