S-adenosyl-L-homocysteine : a non-cytotoxic hypomethylating agent

The cytotoxic effect caused by the hypomethylating agent S-adenosyl-L- homocysteine (SAH) was compared with that of two drugs commonly used to induce DNA hypomethylation, 5-azacytidine and 5-aza-2'-deoxycytidine. Two in vitro cytotoxicity tests, the tetrazolium MTT assay and the intracellular l...

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Veröffentlicht in:	Experientia 1994-07, Vol.50 (7), p.658-659
Hauptverfasser:	DE CABO, S. F, HAZEN, M. J, MOLERO, M. L, FERNANDEZ-PIQUERAS, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Azacitidine - analogs & derivatives Azacitidine - toxicity Biological and medical sciences Cell Membrane Permeability - drug effects Cells, Cultured Decitabine Diverse techniques DNA - metabolism DNA Modification Methylases - antagonists & inhibitors DNA Replication - drug effects Fibroblasts - drug effects Fibroblasts - metabolism Fundamental and applied biological sciences. Psychology Humans L-Lactate Dehydrogenase - metabolism Methylation Mitochondria - drug effects Molecular and cellular biology S-Adenosylhomocysteine - metabolism S-Adenosylhomocysteine - toxicity Tetrazolium Salts
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Zusammenfassung:	The cytotoxic effect caused by the hypomethylating agent S-adenosyl-L- homocysteine (SAH) was compared with that of two drugs commonly used to induce DNA hypomethylation, 5-azacytidine and 5-aza-2'-deoxycytidine. Two in vitro cytotoxicity tests, the tetrazolium MTT assay and the intracellular lactate dehydrogenase (LDH) activity test, suggest that SAH induces hypomethylation without causing any cytotoxic effect. We propose the use of SAH as a non-cytotoxic agent which may be more suitable for inducing experimental DNA hypomethylation.
ISSN:	0014-4754 1420-9071
DOI:	10.1007/BF01952867