Colony hybridization method for screening in situ of eukaryotic amplified genes

A new method for autoradiographic screening of amplified genes in cellular clones is described. The main feature of the device is to keep viable cells from each clone, which can subsequently be regrown. The availability of this biochemical screening method allows screening for recombinants harboring...

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Veröffentlicht in:	Experientia 1984-05, Vol.40 (5), p.483-485
Hauptverfasser:	MALPIECE, Y, MICHEL, M. L, CARLONI, G
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals autoradiography Biological and medical sciences cloning vectors Diverse techniques DNA, Viral - genetics eukaryotes Fundamental and applied biological sciences. Psychology Gene Amplification Genetic Engineering - methods Hepatitis B virus - genetics L Cells (Cell Line) Mice Molecular and cellular biology Nucleic Acid Hybridization
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Beschreibung
Zusammenfassung:	A new method for autoradiographic screening of amplified genes in cellular clones is described. The main feature of the device is to keep viable cells from each clone, which can subsequently be regrown. The availability of this biochemical screening method allows screening for recombinants harboring unselectable markers as well.
ISSN:	0014-4754 1420-9071
DOI:	10.1007/BF01952397