A simplified method for isocratic HPLC analysis of polyamines

A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12-15 min per sample with sensitivity in the pm...

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Veröffentlicht in:	Experientia 1985-09, Vol.41 (9), p.1209-1211
Hauptverfasser:	WHITMORE, W. L, SLOTKIN, T. A
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography, High Pressure Liquid - methods Chromatography, Ion Exchange Fundamental and applied biological sciences. Psychology Intermediary metabolites. Miscellaneous Other biological molecules Polyamines - analysis Putrescine - analysis Rats Spermidine - analysis Spermine - analysis Tissue Distribution
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Beschreibung
Zusammenfassung:	A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12-15 min per sample with sensitivity in the pmole range.
ISSN:	0014-4754 1420-9071
DOI:	10.1007/BF01951733