Effects of ionizing radiations on human costal cartilage and exploration of the procedures to protect the tissue from radiation damage

The chemical changes produced following 60Co-gamma-irradiation of human costal cartilage have been monitored using "critical electrolyte concentration" (CEC) measurements utilizing Alcian Blue--magnesium chloride and Toluidine Blue--sodium chloride systems. The decrease in the CEC with radiation can be related to the decrease in hexose and hexosamine contents of the cartilage. Such changes arise as a result of degradation of the glycosaminoglycan component of the tissue. The state of the collagen fibres after irradiation has been examined using the Van Giesson stain and by biochemical assay of the hydroxyproline moeity. Energy transfer methods have been utilized to protect the glycosaminoglycan component of the cartilage from radiation damage.